EVALUATION OF IMMUNOMODULATORY ACTIVITY OF CRATAEVA NURVALA

Abstract

The body's immune system is one of its most intricate biological systems. An alteration in the strength of the immune response is referred to as immunomodulation. A wide range of allopathic medications are used to alter the immune system. But these medications are prohibitively expensive for the underprivileged, difficult to obtain, and frequently linked to negative drug reactions. Therefore, using herbal formulation as a positive immunomodulator may be advised. Thus this study aims at evaluation of immunomodulatory activity of crataeva nurvala. The plant material was gathered and proccesed properly. Further the extraction ohytochemical and quantitative study along with assessment of parameters associated with immunomodulatory activity. The % loss after drying was estimated to be 30%. After performing defatting and extraction % yield was also calculated. The percentage yield for pet ether and hydroalcoholic extract was found to be 4.4% and 7.2% respectively. The phytochemical screening suggested the presence of alkaloid, flavonoid, saponin, phenolics, protein and carbohydrates. Total phenol & flavonoid content was estimated to be 0.647 and 0.801 respectively. It was observed that, in case of animals treated with HACN 50 and 100mg/kg the % neutrophil adhesion was 46.7 ± 1.2 % and 32.7± 2.0 %respectively which is comparable to that of standard drug Levamisole (0.68 mg/kg) with 38.55 ± 0.6 neutrophil adhesion. The phagocytic index for HACN 50 mg/kg and 100 mg/kg was observed to be 0.0468 ± 0.0027 and 0.0415 ± 0.0016 respectively. The Heamagglutination titer was observed to be 0.0017 ± 0.0003 and 0.0043 ± 0.0008 in HACN 50 mg/kg and 100 mg/kg treated rats. The mice lethality test showed that mortality % 66.66 in plant extract treat group which is equal to the animals treated with drug. Thus it can be inferred that Crataeva nurvala possesses potent immunomodulatory activity.

Keywords: Immunomodulatory, Medicinal plants, Crataeva nurvala, Heamagglutination, mice lethality test, HACN (Hydroalcoholic extract of , Crataeva nurvala), Phytochemicals