FORMULATION AND CHARACTERIZATION OF DOUBLE WALLED MICROSPHERES FOR TREATMENT OF AMOEBIASIS AND GIARDIASIS
Abstract
Entamoeba histolytica infects both small intestine and colon causing giardiasis and amoebiasis respectively. Currently available nitroimidazoles like metronidazole get completely absorbed from upper part of GIT and only negligible part of metronidazole reaches to the small intestine and colon; hence metronidazole is less effective as luminicidal agent. To overcome this problem, double walled microspheres were formulated such that metronidazole should be released in small intestine and colon in effective concentration. Firstly alginate core was formulated and then it was coated with Eudragit L 100. The entrapment efficiency, size and percentage yield of both alginate core and Eudragit shell was calculated. The release kinetics were performed at four different pH conditions like pH 1.2, pH 5.8, pH 6.8 and pH 7.4 by considering GIT conditions and GIT transit time. The drug release study showed that metronidazole was released in both small intestine and colon in effective concentration.
References
Wright S. Amoebiasis and Giardiasis, Medicine 2005;33(8):47-50.
Jalil R, Nixon JR. Microencapsulation using poly(L-lactic acid). IV. Release properties of microcapsules containing phenobarbitone, J. Microencapsul 1990; 7(1):53–66.
Bodmeier R, McGinity JW. The preparation and evaluation of drug containing poly (D,L-lactide) microspheres formed by the solvent evaporation, Pharm. Res. 1987;4:465–471.
Pekarek KJ, Jacob JS, Mathiowitz E. One-step preparation of double-walled microspheres, Adv. Mater. 1994;6:684–686.
Lee HK, Park JH, Kwon KC. Double-walled microparticles for single shot vaccine, J. Cont Rel 1997;44:283–293.
Wang FJ, Wang CH. Sustained release of etanidazole from spray dried microspheres prepared by non-halogenated solvents, J. Cont Rel 2002;81:263–280.
Langer R, Mathiowitz E. Preparation of multiwall polymeric microcapsules, US Patent 4861627, 1989.
Pekarek KJ, Jacob JS, Mathiowitz E. Double-walled polymer microspheres for controlled drug release, Nature 1994;367:258–260.
Rahman Z, Kohli K, Khar RK et al. Charectarization of 5- flurouracil micro-spheres for colonic delivery, AAPS Pharmscitech 2006;7(2):E113-E121.
