QUANTIFICATION, CHARACTERIZATION AND IDENTIFICATION OF PROTEINS IN DIFFERENT VACCINES SAMPLE

Abstract

Aims and Objective

Estimation of protein concentration in Vaccine by Lowry’s method.

Estimation of polysaccharide concentration in Vaccine by Orcinol  method.

 Determination of purity of Vaccine by SDS-PAGE.

                             * Coomassie Staining.

                             *  Silver Staining.

 Identification of Vaccine components  by Western blot method

CONCLUSION

  Quantification of protein is done by Lowry’s Assay.

Correlation factor is 0.997.

  Characterization  of polysaccharide is done by Orcinol Assay.

Correlation  factor is 0.998.

•  SDS-PAGE 
• In Coomassie staining band shows in between 35-45kD marker.
• Silver Staining is more sensitive which also shows its band in between 35-45kD  marker.
• In Western Blotting the markers are more specific which confirms that the molecular weight of sample is 40kD.