SIMULTANEOUS DETERMINATION OF AYURVEDIC HERBS FOR PRELIMINARY SCREENING AND QUALITY CONTROL THROUGH HPTLC

Abstract

Objective: To perform simultaneous estimation of four Ayurvedic herbs for preliminary screening and quality control through HPTLC

Method: Simple and precise HPTLC methods was used for simultaneous determination of four Ayurvedic herbs viz., Foeniculum vulgare, Syzygium aromaticum, Trachyspermum ammi, and Cinnamomum zeylanicum. HPTLC profiling was performed over precoated TLC plates (60 F254, 20 cm

× 10 cm, 250 μm thickness, Merck, Darmstadt, Germany) via a linear ascending technique using Toluene: Ethyl Acetate (9:1 v/v) as mobile phase and derivatization is performed using anisaldehyde sulphuric acid reagent. Detection was achieved at 254 nm, 366nm and 545 nm through spectro-densitometric analysis.

Results: Results of the experiments conducted provided diagnostic characteristics to identify and standardize the raw herbs and Ayurvedic formulations like Mahasudarshan churna. A compact band was obtained for Foeniculum vulgare at Rf value of 0.42 and 0.48 at 254 nm and Rf value of 0.28 and 0.39 was obtained after derivatization with Anisaldehyde sulphuric acid reagent. Similarly results for Syzygium aromaticum, Trachyspermum ammi, and Cinnamomum zeylanicum are mentioned in table 1.

Conclusion: The present investigation would serve as a document to control the quality of raw material like Foeniculum vulgare, Syzygium aromaticum, Trachyspermum ammi, and Cinnamomum zeylanicum and traditional Ayurvedic formulation like Mahasudarshan Churna in various herbal industries.

Keywords:

HPTLC, Quality Control, Raw Herbal Material, Ayurvedic Formulation.