ANTI-OXIDANT STUDIES ON KSHEERAPAKA OF FRESH AND DRIED ROOTS OF BALA (SIDA CORDIFOLIA. L) IN SHSY-5Y CELL MODEL OF NEURODEGENERATIVE DISEASES

Abstract

Background: Neurodegenerative diseases are characterized by progressive neuronal loss, often exacerbated by oxidative stress. Traditional Ayurvedic formulations like ksheerapaka (medicated milk decoctions) have been explored for their therapeutic potential.

Objective: This study evaluates the antioxidant potential of ksheerapaka prepared from fresh and dried roots of Bala (Sida cordifolia L.) using in vitro activities.

Methods: Antioxidant activity was assessed through DPPH scavenging, nitric oxide scavenging, and superoxide anion scavenging activities. The capability of ksheerapaka to reduce Reactive Oxygen Species (ROS) was evaluated using the DCFDA assay in SH-SY5Y neuroblastoma cell lines. Statistical analysis was conducted to compare the activity of ksheerapaka prepared from fresh and dried roots of Bala (Sida cordifolia.L)

Results: Both preparations demonstrated concentration-dependent antioxidant activity, with the ksheerapaka prepared from dried root of Bala (Sida cordifolia.L) showing significantly higher efficacy (p < 0.001) across all assays. The DCFDA assay for invitro ROS measurement further revealed a considerable reduction in ROS levels by the dried root preparation.

Conclusion: The potent antioxidant activity of ksheerapaka, prepared from dried roots of Bala (Sida cordifolia.L), highlights its potential as a neuroprotective agent in mitigating oxidative stress implicated in neurodegenerative diseases. These findings warrant further in vivo and clinical studies to explore its therapeutic activity.