A COMPARATIVE ANALYSIS OF HARIDRA CHURNA (CURCUMA LONGA LINN.) AND AMALAKI (EMBLICA OFFICINALIS GAERTN.) SWARASA BHAVITA HARIDRA (CURCUMA LONGA LINN) CHURNA THROUGH HIGH PERFORMANCE THIN-LAYER CHROMATOGRAPHY

Abstract

Introduction: High-Performance Thin-Layer Chromatography (HPTLC) is an advanced version of Thin-Layer Chromatography (TLC) used for the separation, identification, and quantification of compounds in a sample. This analytical technique employs a stationary phase, which consists of a thin layer of adsorbent (typically silica gel or alumina) coated on a flat surface like a glass, plastic, or aluminium plate, along with a mobile phase that is a solvent or a mixture of solvents. HPTLC has a wide range of applications across various industries, including quality control, the quantification and detection of adulterants, authenticity and purity testing, additive analysis, and drug analysis. Its sensitivity, versatility, and capability to analyze complex mixtures make it a valuable tool in these fields.

Materials and Methods: Haridra churna (powder of Curcuma longa Linn.) and Amalaki (Emblica officinalis Gaertn.) swarasa bhavita haridra (Curcuma longa Linn) churna were prepared using the same methodology for both preparations. The HPTLC fingerprinting of both samples was conducted using a methanolic extract. The analysis of the bands was performed at wavelengths of 254 nm and 366 nm using the CAMAG Linomat V Automatic Sample Spotter. The peaks and areas were compared for evaluation.

Results and Discussion: HPTLC chromatogram of methanolic extracts of churna and bhavita churna showed 9 peaks at 254nm with a total area of 77202.6 AU and 18549 AU respectively. At 366 nm churna showed 5 peaks with a total area of 51440.9AU and bhavita churna showed 8 peaks with a total area of 49365.9 AU.

Conclusion: At 254 nm, both churna and bhavita churna showed the same number of peaks. However, bhavita churna exhibited a greater number of larger value peaks at 366 nm, indicating that bhavita churna is more potent compared to churna.