ESTIMATION OF ANTI-MICROBIAL ACTIVITY OF CISSUSS QUADRANGULARIS AQUEOUS EXTRACT
Abstract
The Cissus quadrangularis is having anti-microbial activity and every part of this plant is important in medicine and various preparations in indigenous system of remedy for Cissus quadrangularis is used for diabetes, obesity, high cholesterol, bone fractures, allergies, cancer, stomach upset, painful menstrual periods, vaginal infections, asthma, malaria, wound healing, peptic ulcer disease, osteoporosis. In this study the in vitro anti-microbial activity of aqueous extract is tested against gram-positive, gram-negative organism’s and fungi strains by agar disc diffusion method. The crude extract showed a broad spectrum of anti-microbial activity by inhibiting both the gram positive, gram negative, fungi groups. The anti-microbial activity of C. quadrangularis plant extract using solvents such as water was evaluated against the gram-positive strains such as St. aureus, B. substilis and in gram negative strains such as E. coli (ETEC), P. aeruginosa and fungi such as Saccharomyces cerevisiae (Yeast), Candia albicans. Each test organism was also studied by observing their growth in presence of the aqueous extract at various incremental levels, equivalent to 25µg/mL- 800µg/mL. The highest activity was observed in aqueous extract against gram positive and gram-negative organism’s and fungi with an inhibitory concentration < 50 µg/ml. The significance of the study was conducted to examine the in vitro anti-microbial activity of C. quadrangularis plant and to evaluate their medicinal proprties.
Keywords: Anti-microbial, Cissus quadrangularis, disc plate method, Minimal inhibitory concentration (MIC)
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