ANTI-INFLAMMATORY POTENTIAL OF α-FENCHOL AND αGURJUNENE: AN IN VITRO STUDY

Mithun Singh Rajput, Devashish Rathore, Rashmi Dahima

Abstract


Chronic inflammatory diseases are still one of the main health complications acknowledged globally. At present, although synthetic drugs are dominating the market, element of toxicity that these drugs entail, cannot be ruled out; hence the search for newer therapeutic strategies with lesser side effects has always been a matter of research. The present study aims to evaluate the in vitro anti-inflammatory effects of two essential oils α-Fenchol and α-Gurjunene. Methodology: In vitro anti-inflammatory activity of both the essential oils was evaluated using albumin denaturation assay. Results:  At various concentrations viz. 31.25, 62.50, 125.00, 250.00, 500.00 and 1000.00 μg/ml, α-Fenchol exhibited inhibition of protein denaturation in the range of 180-1400% and α-Gurjunene in the range of 290-360%. The present findings corroborated the anti-inflammatory property of essential oils, α-Fenchol and αGurjunene in vitro.  Conclusion: Both the essential oils possess in vitro anti-inflammatory effects, further definitive studies are necessary to ascertain the mechanisms behind their anti-inflammatory actions in vivo using suitable experimental models.   Keywords: α-Fenchol, α-Gurjunene, Anti-inflammatory, Protein denaturation

Full Text:

PDF

References


Vane JR, Botting RM. New insight into the mode of action of anti-inflammatory drugs. Inflamm Res 1995;44:1-10.

Gerard J Tortora, Sandra Reynolds, eds. Principles of Anatomy and Physiology. 7th ed. Harper Collins College Publishers, 1993, pp 695.

Yesilada E, Ustun O, Sezik E, Takaishi Y, Ono Y, Honda G. Inhibitory effect of Turkish folk remedies on inflammatory cytokines: Interleukins-1α, interleukins-1β and tumour necrosis factor-α. J Ethnopharmacol 1997;58:59–73.

Rubiolo P, Sgorbini B, Liberto E, Cordero C, Bicchi C. Essential oils and volatiles: sample preparation and analysis. Flavour Fragr J 2010;25:282-290.

Burt S. Essential oils: their antibacterial properties and potential applications in foods – a review. Int J Food Microbiol 2004;94:223-253.

Cavaleiro, CMF. Óleos essenciais de Juniperus de Portugal. PhD Thesis, Universidade de Coimbra, Faculdade de Farmácia, Coimbra, Portugal, 2001.

Pourmortazavi SM, Hajimirsadeghi SS. Supercritical fluid extraction in plant es-sential and volatile oil analysis. J Chromatogr A 2007;1163:2-24.

Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules 2010;15:9252-9287.

Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of anti-inflammatory effect of ashwagandha: a preliminary study in vitro. Phcog J 2012;4:47-49.

Choi YH, Kim GY, Lee HH. Anti-inflammatory effects of cordycepin in lipopolysaccharide-stimulated raw 264.7 macrophages through toll-like receptor 4-mediated suppression of mitogen-activated protein kinases and NF-kB signaling pathways. Drug Des Devel Ther 2014;8:1941e53.

Opie EL. On the relation of necrosis and inflammation to denaturation of pro-teins. J Exp Med 1962;115:597-608.

Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN, Iputo JE. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med Plants Res 2010;4:789-795.

Williams LAD, O’Connar A, Latore L et al. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (im-munogenic) bovine serum albumin is proposed as a screening assay for the de-tection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med J 2008;57:327-31.

Ghosh AK. Banarjee M, Bhattacharyya NK. Anti-inflammatory activity of root of Alpinia galanga willd. Chron Young Sci 2011;2:139-43

Kumar S, Tiwari R, Alam N. anti-inflammatory activity of methanolic extract of Cyperus rotundus rhizome on carrageenan induced paw edema in rats. Int J Pharm Sci Res 2012;12:5097-5100.

How to cite this article:

Mithun Singh Rajput, Devashish Rathore, Rashmi Dahima. Anti-inflammatory potential of α-fenchol and α-gurjunene: an in vitro study; Panacea Journal of Pharmacy and Pharmaceutical Sciences 2018:7(3);129-135.


Refbacks

  • There are currently no refbacks.