ROLE OF THE PHYTOESTROGEN DAIDZEIN IN EXPERIMENTALLY INDUCED BREAST CANCER IN WISTAR RATS
Abstract
Background: Phytoestrogens have recently emerged as potential sources of chemo prevention especially in mammary carcinogenesis. Several controversies exist about their role in preventing cancer progression, has propelled a more profound research activity involving these flavonoids . Aims & Objectives: This study was carried out to explore the anti - cancer potential of the isoflavone Daidzein using appropriate animal models, and to evaluate its effect on the oxidative stress induced by 7,12-dimethylbenz (a) anthracene (DMBA) Materials & Methods: DMBA - a chemical carcinogen was administered (25mg/rat/ s.c) in 2 divided doses to induce mammary cancers in female Wistar rats. Daidzein at doses of 50mg/kg and 100mg/kg and Tamoxifen as reference standard (40mg/kg) was administered, starting from the 1st day. The growth of tumors was monitored after the treatment. The mammary tumors collected from rats were analyzed by estimation of anti-oxidant enzymes, 17β estradiol levels to determine their chemo preventive potential. Results: Daidzein at a human equivalent dose suppressed the growth of DMBAinduced mammary tumors especially at higher doses of 100mg/kg, which were comparable to the effects of Tamoxifen (P <0.05). Daidzein showed the potential of inhibiting the growth of mammary tumors in rodents and attenuating oxidative stress by modifying the effect of anti-oxidant enzymes. Conclusion: Daidzein could be used as a core structure to design new drugs for breast cancer therapy. The outcome of results indicates that consumption of Daidzein may protect against breast cancer. Key words: Chemoprevention, Daidzein, DMBA, isoflavone, mammary carcinogenesis, Phytoestrogens, Tamoxifen.References
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