PHARMACEUTICO-ANALYTICAL COMPARISON OF KIRATATIKTHADHI YOGA, TRADITIONAL VERSES SOXHLET EXTRACTION METHOD

Abstract

The current work focuses on the development of Hydro-alcoholic and Aqueous extracts of Kiratatikthadhi yoga, and to compare it with decoction of the same using HPTLC finger printing. Kiratatikthadhi yoga is a traditional Ayurvedic formulation consisting of five herbal ingredients: Kiratatiktha, Katurohini, Musta, Parpataka, and Amrutha which is mentioned in the Charaka Samhita Chikitsa Stana as a remedy for Punaravarthaka Jwara, a specific type of fever. In the light of recent amendments to the Drugs and Cosmetics Rule 158 B clause IV and the guidelines of the Ayurvedic Pharmacopoeia of India, the study explores newly introduced Kalpana of Hydro-alcoholic and Aqueous extracts of Kiratatikthadhi yoga. To achieve this, a Soxhlet apparatus was used to prepare the extracts and followed contemporary method of kwatha nirmana to prepare the traditional decoction. The resulting products were converted into a powdered form using a tray drying method after incorporating an appropriate amount of fine powder from the same formulation. The quantity of fine powder required for this drying process was standardized beforehand. Subsequently, a comparative analysis was conducted between the extracts and the decoction using High-Performance Thin-Layer Chromatography (HPTLC) fingerprinting. The final results demonstrated that the powdered extracts exhibited higher content of actives than the powdered kasaya, as evidenced by a greater number of peaks observed in the HPTLC analysis. Furthermore, the physicochemical analysis of both the extracts and the decoction showed similar ranges of values.

Keywords: Hydro-alcoholic extract, Aqueous extract, kiratatikthadhi yoga, Soxhlet apparatus, tray drying, High-Performance Thin-Layer Chromatography.