STUDY OF PHYTOCHEMICALS AND STZ INDUCED ANTIDIABETIC ACTIVITY OF LITSEA GLUTINOSA

Abstract

Based on recent estimates, 438 million adults (7.8% of the adult population) are expected to have diabetes by 2030. The diabetes drugs that are currently available have a lot of unfavourable side effects. The plant Litsea glutinosa is used traditionally for many alignments and has several medicinal properties.  This study aims to analyse Litsea glutinosa's anti-diabetic effect. Following the collection of the plant material, it underwent both qualitative and quantitative analysis and hydroalcoholic extraction. Next, using diabetes rats caused by STZ, the in vivo antidiabetic investigation was conducted. The hydroalcoholic bark extract of Litsea glutinosa was administered to certain group of rats in 100mg/kg & 200mg/kg of concentration. Standard protocol was to utilise glibenclamide. The presence of alkaloids, carbohydrates, glycosides, saponins, phenols, flavonoids, and proteins were detected by phytochemical screening, according to the results. It was discovered that the total concentration of phenol and alkaloids was 0.417 and 0.954 mg/100 mg. The blood glucose levels measured on the 21st day were 214.1±1.52 & 170.8±1.66 mg/dl in 100 mg/kg & 200 mg/kg, respectively. Glibenclamide, on the other hand, showed an observation of 150.3±1.85 mg/dl.  Rats given 100 mg/kg and 200 mg/kg extract had total cholesterol levels of 124.0 ± 1.50 & 109.1 ± 1.30 mg/dL, respectively. The total cholesterol concentration in the blood of rats treated with Glibenclamide was found to be 101.4 ± 1.10. Additionally, the rats given 100 mg/kg and 200 mg/kg extract were found to have triglyceride levels of 124.00 ± 1.80 & 114.00 ± 1.80 mg/dL, respectively. In the 200 mg/kg extract group, the observed protein content was 7.43 ± 0.60 g/dL. Rats in a 200 mg/kg hydroalcoholic bark extract of Litsea glutinosa were found to weigh 185.00 ± 1.40 in the end. In conclusion, the blood glucose levels seen following the administration of Listea glutinosa hydroalcoholic extract to rats induced with diabetes were comparable to those observed following glibenclamide treatment.

Keywords: Diabetes, Medicinal plants, Phyochemicals, Listea glutinosa, STZ induced diabetes, Glibenclamide