A COMPARATIVE ANALYSIS OF HPTLC FINGER PRINTING PROFILE OF CHOORNA AND BHAVITHA CHOORNA OF APAMARGA (ACHYRANTHES ASPERA LINN.)
Abstract
Samskara is a pharmaceutical process which aids in the transformation of the inherent attributes of a dravya which provides to the addition of a new property in them. The samskara or the processing helps in the qualitative and quantitative alterations for the improvement, intensification, modification and lowering the unwanted effects of the substance. Bhavana is one mode of samskara in which the powdered drugs are soaked in an appropriate drava (liquid medium like swarasa, kashaya etc.). Dravya is kept completely immersed in the drava or it is triturated in the drava for specific time period. The drug Apamarga (Achyranthes aspera Linn.) is selected as the drug for choice for the purpose of bhavana. The present study was aimed to compare the HPTLC finger printing profile of choorna and bhavitha choorna of the drug. Test solution is made with 10.0760gm of choorna and 10.4896 gm bhavitha choorna of Apamarga (Achyranthes aspera Linn.) respectively. Both the powder of the drug was then extracted with 1 ml methanol and 10 μl of each was applied on the stationary phase Silica gel 60 F254. Mobile phase selected was toluene: ethyl acetate: methanol (6:3.8:0.2). The development of the plate was done by using CAMAG 20 x 10 cm automatic developing chamber and was visualized under UV at 254 nm and 366 nm after derivatization using anisaldehydesulphuric acid. The results revealed that there obtained different peak intensities in both sample but peaks with similar Rf values in both the choorna was noticed which indicates the presence of same chemical constituent. Also the maximum peak area AU obtained for similar Rf values in the bhavitha choorna were more compared to the choorna indicating the amplification of phytoconstituents in the bhavitha choorna by the process of bhavana.
Key words: Apamarga, samskara, bhavana, choorna, bhavitha choorna, HPTLC finger printing profile
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