SYSTEMATIC REVIEW ON THE COMPARISON OF PRACTICALITY OF H9C2 AND HL-1 CELL LINE INVITRO MODELS IN TESTING THE ROS SCAVENGING ACTIVITY OF AYURVEDIC CARDIOPROTECTIVE MEDICINES
Abstract
A new initiative “Global Hearts” launched by WHO aims to beat back the global threat of cardiovascular diseases – the leading cause of death worldwide. By analysing different cardiovascular diseases (CVDs) the main pathophysiology behind is; oxidative stress generated by the increased production of reactive oxygen species (ROS). The ROS scavenging activity of medicines can be tested through invitro research. H9C2 cell line and HL-1 cell line are popularly used invitro models for testing the cardiotoxic and cardioprotective study of medicines. The administration of antioxidants helps to scavenge these ROS and can be utilized as both preventive and curative aspect of cardiovascular diseases. In Ayurveda, many single drugs and polyherbal preparations are mentioned in hridroga chikilsa (treatment for heart diseases) with a natural antioxidant supply. Among them, some were tested through invitro studies. This systematic review tries to compare the practicality of H9C2 cell line and HL-1 cell line invitro models in testing the ROS scavenging activity of Ayurvedic cardioprotective medicines and this review was completed by the comprehensive analysis of published research works in PubMed database and google scholar. The review of the literature was arranged systematically, guided by the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) flow diagram. The review was concluded by comparing the number of available research works on both cell line invitro models used in the study of Ayurvedic cardioprotective medicines. The H9C2 cell line model was found to be more utilized for testing the ROS scavenging activity of Ayurvedic cardioprotective medicines.
KEYWORDS: H9C2 cell line, HL-1 cell line, Invitro study, Reactive oxygen species, Ayurvedic medicine
References
https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)
Demetrios Moris.et al. The role of reactive oxygen species in the pathophysiology of cardiovascular diseases and the clinical significance of myocardial redox.Annals Of Translational Medicine.2017,August;5(16):326
Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal.2012,May;24(5):981-90
SL.Nuttal etal. Antioxidant therapy for the prevention of cardiovascular disease.
QJM: An International Journal of Medicine.1999,May; 92(5):239–244
Piotr Witek et al. The effect of a number of H9C2 rat cardiomyovytes passage on repeatability of cytotoxicity study results.Cytotechnology.2016,Dec;68(6):2407-2415
Asensio-López MC, Soler F, Pascual-Figal D, Fernández-Belda F, Lax A. Doxorubicin-induced oxidative stress: The protective effect of nicorandil on HL-1 cardiomyocytes. PLoS One.2017, Feb ,28;12(2):e0172803
William C Claycomb.et al.HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte.PNAS. 1998, March,17; 95 (6): 2979-2984
Ashutosh Chauhan, Deepak Kumar Semwal , and Ruchi Badoni Semwal. Ayurvedic research and methodology: Present status and future strategies. Ayu.2015, Oct-Dec; 36(4): 364–369
https://www.who.int/medicines/publications/traditional/trm_strategy14_23/en/
Kuznetsov AV, Javadov S, Sickinger S, Frotschnig S, Grimm M. H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochim Biophys Acta. 2015 Feb;1853(2):276-84.
http://prisma-statement.org/prismastatement/Checklist.aspx
Srimachai S, Devaux S, Demougeot C, Kumphune S, Ullrich ND, Niggli E, Ingkaninan K, Kamkaew N, Scholfield CN, Tapechum S, Chootip K. Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury. BMC Complement Altern Med.2017 ,Feb ,20;17(1):117
Qinghua Zeng etal. 20-HETE increases NADPH oxidase-derived ROS production and stimulates L type calcium channel via PKC-dependent mechanism in cardiomyocytes. Am J Physiol Heart Circ Physiol.2010,July,30;299:H1109-H1117
Prathapan A, Vineetha V, Abhilash P & Raghu K. Boerhaavia diffusa L.attenuates angiotensin II- induced hypertrophy in H9C2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-kβ and transforming growth factor β1.British Journal Of Nutrition.2013,April,16;110(7):1201-1210
Maitinuer Maiwulanjiang etal. The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte.Journal of Ethnopharmacology.2014; 153( 2): 491-498
R. Lakshmi Sundaram, Hannah R. Vasanthi. Dalspinin isolated from Spermacoce hispida (Linn.) protects H9c2 cardiomyocytes from hypoxic injury by modulating oxidative stress and apoptosis.Journal of Ethnopharmacology.2019;241:111962
Shishir Upadhyay, Anil Kumar Mantha, Monisha Dhiman. Glycyrrhiza glabra (Licorice) root extract attenuates doxorubicin-induced cardiotoxicity via alleviating oxidative stress and stabilising the cardiac health in H9c2 cardiomyocytes. Journal of Ethnopharmacology. 2020, August,10;258:112690
Wang, WC.,Uen, YH.,Chang,ML. et al. Protective effect of guggulsterone against cardiomyocyte injury induced by doxorubicin invitro.BMC Complement Altern Med.2012,August,27;12:138
L.Chularojmontri, O.Gerdprasert, S.K. Wattanapitayakul. Pummelo Protects Doxorubicin-Induced Cardiac Cell Death by Reducing Oxidative Stress, Modifying Glutathione Transferase Expression, and Preventing Cellular Senescence. Evidence-Based Complementary and Alternative Medicine.2013,Jan,21;13:9
Barot, Bhavya N.Therapeutic Potential of Fermented Papaya Preparation in Doxorubicin Induced Toxicity.Maharaja Sayajirao University of Baroda (India), ProQuest Dissertations Publishing.2017. 27738802.
Shreesh Ojha, Hasan Al Taee, Sameer Goyal, Umesh B. Mahajan, Chandrgouda R.Patil, D. S. Arya, Mohanraj Rajesh. Cardioprotective Potentials of plant-Derived Small Molecules against Doxorubicin Associated Cardiotoxicity. Oxidative Medicie and Cellular Longevity.2016,May,23;vol 2016:19
Hardik Savsani, Abhay Srivastava, Sarita Gupta, Kirti Patel.Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study,Heliyon.2020.Jan;6(1)
Desai, Kumariswati N.A Study on Anethum graveolens L. Seed Extract in Alleviating Cardiovascular Complications using in vitro and in vivo Experimental Models. Maharaja Sayajirao University of Baroda (India), ProQuest Dissertations Publishing. 2017. 27672487.
Ramakrishnan Ganapathy .etal. Thraatchathi Chooranam, protects cardiomyocytes against oxidative stress. Frontiers In Bioscience, Elite.2018,march,1;10:437-448
Ganapathy, R., Ramachandran, A., Shivalingaiah, S.B. et al. Cardioprotective potential of polyphenols rich Thraatchathi Chooranam against isoproterenol induced myocardial necrosis in experimental rats. BMC Complement Med Ther 20, 356 (2020).
Hamid Mollazadeh.etal.Effects of pomegranate seed oil on oxidant/antioxidant balance in heart and kidney homogenates and mitochondria of diabetic rats and high glucose-treated H9c2 cell line. Avicenna J Phytomed. 2017 Jul-Aug; 7(4): 317–333
Balkrishna A, Rustagi Y, Bhattacharya K, Varshney A. Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras. Biomolecules.2020, April, 13;10(4): 600
P.L. Reshma et al. Pretreatment of Tribulus terrestris L. causes anti-ischemic cardioprotection through MAPK mediated anti-apoptotic pathway in rat. Biomedicine & Pharmacotherapy.2019, March; 111:1342-1352
