EFFECT OF SHAD RITU ON COLLECTION OF MANDUKPARNI ON PUSHYA NAKSHATRA

Abstract

This study explores the seasonal variations in the physico-chemical and phyto-chemical properties of Mandukaparni (Centella asiatica), a widely used medicinal plant in Ayurveda. Six different seasons (Ritus)—Vasant, Grishma, Varsha, Sharad, Hemant, and Shishir—were examined to understand how environmental changes affect the plant's chemical composition. Key physico-chemical parameters, including pH, total ash content, acid insoluble ash, loss on drying, water-soluble extractives, alcohol-soluble extractives, and triterpenoid content, were analyzed across the seasons. Additionally, phyto-chemical identification of alkaloids, glycosides, flavonoids, tannins, steroids, terpenoids, saponins, carbohydrates, and proteins was performed. HPTLC analysis at 254 nm, 366 nm, and 540 nm wavelengths was also conducted to detect variations in the plant’s phytochemical profiles. The results indicate that while certain parameters, such as triterpenoid concentration and water-soluble extractive values, are higher in winter (Shishir Ritu), the overall chemical profile of Mandukaparni remains relatively consistent throughout the year. These findings suggest that optimal harvesting for specific bioactive compounds may vary by season, with winter providing the highest concentrations of key medicinal constituents. The research provides a comprehensive understanding of the influence of seasonal variation on Mandukaparni's therapeutic efficacy, aiding in better harvesting and utilization practices.

KEYWORDS- Mandukaparni, Centella asiatica, physico-chemical analysis, phyto-chemical constituents, HPTLC analysis, Ritus