RP-HPLC ANALYSIS OF CHLORANTRANILIPROLE PESTICIDE IN CAULIFLOWER VEGETABLE AT HESARAGHATTA VILLAGE BENGALURU

Ambujakshi H R, Anirbandeep Bose, Selvakumar K, Akash Gupta, Hemalatha Y R

Abstract


The present work is aimed to develop a simple and rapid RP-HPLC method for the determination of chlorantraniliprole pesticide residue in cauliflower vegetables collected from different markets at Hesaraghatta village Bengaluru. The QuEChERS extraction method was employed to extract pesticides. The chromatographic separation was carried out using C18 column with mobile phase consisting of acetonitrile:water (40:20 v/v) at a flow rate of 0.6 ml/min. The chromatographic peak was measured at 254 nm. The obtained chromatogram showed a well-resolved chlorantraniliprole peak within run time of 4.30 min. The proposed method was validated as per US-FDA guidelines and found to be linear, accurate, precise, selective and robust. The proposed method was applied for the estimation of chlorantraniliprole pesticide residue in real sample. There was no chlorantraniliprole pesticide residue peak was observed. The study revealed the safety consumption of cauliflower vegetables. The proposed method can be adopted for the routine analysis of chlorantraniliprole pesticide residue in cauliflower vegetables.

Keywords: Chlorantraniliprole, Cauliflower, HPLC, QuEChERS extraction method, US-FDA guidelines


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How to cite this article:

Ambujakshi H R, Anirbandeep Bose, Selvakumar K, Akash Gupta; RP-HPLC Analysis of chlorantraniliprole pesticide in Cauliflower vegetable at Hesaraghatta village Bengaluru; Panacea Journal of Pharmacy and Pharmaceutical Sciences 2018:7(3);71-82.


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