STUDY OF PHYTOCHEMICALS AND ANTI ACNE ACTIVITY OF ETHANOLIC EXTRACT OF WRIGHTIA TINCTORIA
Abstract
Acne vulgaris, a multifarious chronic inflammatory state and mostly happen within a pilosebaceous unit including hair, hair follicles, sebaceous gland of the skin. Acne is categorized by non-inflammatory lesions that are whiteheads, blackheads, and inflammatory lesions that are papules, nobles, pustule, podules, and cysts. The most common form of the disease in adolescents in is called acne vulgaris. Patients experience psychological burdens like depression, anxiety, and low self-esteem because of acne The therapies which are commonly used to the treatment of Acne vulgaris include are hormonal, systemic, topical, herbal and some drugs with combination therapy But, the use of these drugs produces a numeral of potential side effects and consumption of antibiotics will usually make the bacteria become resistant and permanent nature multiplies within its host. Herbal therapies, which have been in use from ancient times for the treatment of acne, include various herbal extracts, oil and their ayurvedic Wrightia tinctoria which is commonly known as ‘Danthapala’ is a known potential medicinal plant, the leaves of which is traditionally used in the treatment of psoriasis and non-specific dermatitis in Siddha and Ayurvedic systems of medicine formulation. So the aim of present study is to evaluate phytochemicals and anti acne effect of Wrightia tinctoria . The leaves of plant were collected and subjected to extraction by pet ether & ethanol. Further qualitative & quantitative estimation of phytochemical was performed. The antimicrobial activity was also evaluated.
The results showed that the percentage yield for pet ether extract & Ethanolic extract for leaves of Wrightia tinctoria was found to be 1.52% 7.54% respectively suggesting ethanol as better solvent. The phytochemical screening revealed presence of flavonoid, carbohydrate, proteins & diterpenes. The total flavonoid & total protein content of ethanolic extract of Wrightia tinctoria was found to be 0.762mg/100mg and 0.541 mg/100mg of dried extract. The antimicrobial activity was evaluated by well diffusion method. Four bacterial species Streptococcus mutans, Klebsiella pneumoniae, Candida albicans, Propionibacterium acnes were tested for effect of Wrightia tinctoria extract. At concentration of 100mg/ml the zone of inhibition for Streptococcus mutans, Candida albicans, Propionibacterium acnes was found to be 9±0.94mm, 15±0.47mm, 17±0.74mm respectively. The zone of inhibition were not seen in case of Klebsiella pneumoniae The result of antimicrobial acytivity suggest that the ethanolic extract of Wrightia tinctorial was highly effective against Propionibacterium acnes. From the results it can be conclude that Wrightia tinctoria exhibit significant anti acne potential.
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