STUDY OF PHYTOCHEMICALS AND FORMULATION OF TOPICAL ANTIMICROBIAL GEL OF MEDICINAL PLANTS

Authors

  • Shashi Prakash
  • Anshita Gupta
  • Dr. Vivek Gupta

Abstract

The two plant species Mahonia leschenaultia and Chromolaena odorata are used in this study to formulate antimicrobial topical gel. The leaves of Mahonia leschenaultia and aerial part of Chromolaena odorata were collected and subjected to extraction by hydroalcoholic solvent. The qualitative & quantitative studies on phytochemicals were also performed. The gel was formulated by standard procedures & evaluated for its various parameters along with antimicrobial activity. The result of the study showed that The percentage yields for Mahonia leschenaultia and Chromolaena odorata hydroalcoholic were found to be 8.52 and 7.63 % w/w respectively. The Mahonia leschenaultia extract contains alakaloid, flavonoid, diterpenes, phenol & proteins. While, Chromolaena odorata extract found to have presence of alkaloid, diterpenes , phenol, proteins, carbohydrate & saponins. Total alkaloid & phenol content in Mahonia leschenaultia extract was found to be 0.263 mg/ 100mg & 0.745mg/100mg respectively. In case of Chromolaena odorata total alkaloid & phenol content 0.374 mg/100 mg & 0.695 mg/100mg of dried extract respectively. Antimicrobial activity showed that the formulation HG5 have greater antimicrobial efficacy against E. coli its zone of inhibition of 14±0.57 at 25mg/ml concentration.

Key words: Mahonia leschenaultia and Chromolaena odorata, Phytochemicals Formulation, Antimicrobial Gel.

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Published

2022-12-23

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Original Research Article